Description
The assay utilizes the ability of 21-OH Abs to act divalently, and to form a bridge between immobilized 21-OH and 21-OH-Biotin in the fluid phase. In the first step, 21-OH Ab present in samples binds with 21-OH immobilized onto the microtiter plate. In the second step, 21-OH-Biotin binds to this complex. The amount of 21-OH-Biotin bound correlates with the level of antibodies present in patient samples. Unbound 21-OH-Biotin is then removed by washing. Bound 21-OH-Biotin can then be quantified by addition of streptavidin peroxidase (SA-POD) and a colorigenic substrate (TMB), and reading the optical density at 450nm